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compatible cutters xmai  (New England Biolabs)


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    Structured Review

    New England Biolabs compatible cutters xmai
    Compatible Cutters Xmai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 743 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xmai/Xma+I/bio_rxiv__64898__2026__04__28__721222-260-14-17
    Average 96 stars, based on 743 article reviews
    compatible cutters xmai - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: Functional Study of PgHDZ01 Gene Involved in the Regulation of Ginsenoside Biosynthesis in Panax ginseng .
    Article Snippet: .. The confirmed fragment was then digested with XmaI (New England Biolabs (China, Beijing) Ltd.) and ligated into the similarly digested pCAMBIA3301 expression vector. ..

    Amplification:

    Article Title: The yeast DENN domain protein Avl9 contributes to recycling and sorting of endosomal cargos
    Article Snippet: .. GFP-tagged Rabs were amplified by PCR from their original plasmids using M13 forward and reverse primers, and subcloned by homologous recombination into pRS317 digested with EcoRI and XmaI (New England Biolabs, Ipswich, MA, USA) ( ). .. Fluorescence microscopy was performed using a DMi8 inverted fluorescence microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 100×, 1.47 numerical aperture (NA) Plan-Apochromat oil immersion lens, a Flash 4.0 v3 sCMOS camera (Hamamatsu, Shizuoka, Japan), an LED3 fluorescence illumination system, 488 nm and 561 nm lasers, a W-View Gemini image splitting optical device (Hamamatsu), compatible filter sets for fluorescence and DIC imaging, and LAS X v3.7.6.25997 software (Leica).

    Polymerase Chain Reaction:

    Article Title: The yeast DENN domain protein Avl9 contributes to recycling and sorting of endosomal cargos
    Article Snippet: .. GFP-tagged Rabs were amplified by PCR from their original plasmids using M13 forward and reverse primers, and subcloned by homologous recombination into pRS317 digested with EcoRI and XmaI (New England Biolabs, Ipswich, MA, USA) ( ). .. Fluorescence microscopy was performed using a DMi8 inverted fluorescence microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 100×, 1.47 numerical aperture (NA) Plan-Apochromat oil immersion lens, a Flash 4.0 v3 sCMOS camera (Hamamatsu, Shizuoka, Japan), an LED3 fluorescence illumination system, 488 nm and 561 nm lasers, a W-View Gemini image splitting optical device (Hamamatsu), compatible filter sets for fluorescence and DIC imaging, and LAS X v3.7.6.25997 software (Leica).

    Article Title: Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidney.
    Article Snippet: .. The transgene was digested with KpnI (NEB, #R3142S) and XmaI (NEB, #R0180S), gel-purified to remove plasmid vector sequences using the Wizard SV Gel and PCR Clean-Up System (Promega, #A9281), and further purified by ethanol precipitation. ..

    Article Title: Comprehensive CRISPR/Cas9-based mutagenesis identifies single-amino acid substitutions that abrogate SPEN function in X inactivation.
    Article Snippet: .. PCR product and vector were both digested with NotI-HF (NEB) and XmaI (NEB) according to the manufacturer’s protocol. .. Desired DNA fragments were then extracted using QIAquick Gel Extraction Kit (QIAGEN).

    Homologous Recombination:

    Article Title: The yeast DENN domain protein Avl9 contributes to recycling and sorting of endosomal cargos
    Article Snippet: .. GFP-tagged Rabs were amplified by PCR from their original plasmids using M13 forward and reverse primers, and subcloned by homologous recombination into pRS317 digested with EcoRI and XmaI (New England Biolabs, Ipswich, MA, USA) ( ). .. Fluorescence microscopy was performed using a DMi8 inverted fluorescence microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 100×, 1.47 numerical aperture (NA) Plan-Apochromat oil immersion lens, a Flash 4.0 v3 sCMOS camera (Hamamatsu, Shizuoka, Japan), an LED3 fluorescence illumination system, 488 nm and 561 nm lasers, a W-View Gemini image splitting optical device (Hamamatsu), compatible filter sets for fluorescence and DIC imaging, and LAS X v3.7.6.25997 software (Leica).

    Plasmid Preparation:

    Article Title: Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidney.
    Article Snippet: .. The transgene was digested with KpnI (NEB, #R3142S) and XmaI (NEB, #R0180S), gel-purified to remove plasmid vector sequences using the Wizard SV Gel and PCR Clean-Up System (Promega, #A9281), and further purified by ethanol precipitation. ..

    Article Title: Comprehensive CRISPR/Cas9-based mutagenesis identifies single-amino acid substitutions that abrogate SPEN function in X inactivation.
    Article Snippet: .. PCR product and vector were both digested with NotI-HF (NEB) and XmaI (NEB) according to the manufacturer’s protocol. .. Desired DNA fragments were then extracted using QIAquick Gel Extraction Kit (QIAGEN).

    Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation.
    Article Snippet: .. Plasmids were cut with XmaI (New England Biolabs; # R0180S) and PsiI (New England Biolabs; # R0744S), and a geneblock containing the bsd gene was ordered (gb_bsd) and cloned in the cut vector via Gibson assembly in the purified vector for 3 h at 50◦C followed by DH5 α E. coli competent cells (Thermo Fisher Scientific; #18265017). ..

    Purification:

    Article Title: Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidney.
    Article Snippet: .. The transgene was digested with KpnI (NEB, #R3142S) and XmaI (NEB, #R0180S), gel-purified to remove plasmid vector sequences using the Wizard SV Gel and PCR Clean-Up System (Promega, #A9281), and further purified by ethanol precipitation. ..

    Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation.
    Article Snippet: .. Plasmids were cut with XmaI (New England Biolabs; # R0180S) and PsiI (New England Biolabs; # R0744S), and a geneblock containing the bsd gene was ordered (gb_bsd) and cloned in the cut vector via Gibson assembly in the purified vector for 3 h at 50◦C followed by DH5 α E. coli competent cells (Thermo Fisher Scientific; #18265017). ..

    Ethanol Precipitation:

    Article Title: Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidney.
    Article Snippet: .. The transgene was digested with KpnI (NEB, #R3142S) and XmaI (NEB, #R0180S), gel-purified to remove plasmid vector sequences using the Wizard SV Gel and PCR Clean-Up System (Promega, #A9281), and further purified by ethanol precipitation. ..

    Incubation:

    Article Title: 4-Hydroxyphenanthrene exacerbates obesity by altering gut microbiota and bile acid metabolism.
    Article Snippet: In general, 1000 bp of the hcp upstream gene, xylan-inducible promoter and hcp gene with xmaI and claI restriction enzymes were synthesised and combined in the pUC57 vector. pLGB31 was purchased from Addgene. .. Briefly, 4 μg pUC57 and 4 μg pLGB31 were incubated with 1 unit xmaI (NEB, UK) and 1 unit claI (NEB, UK) in 50 μL reaction volume at 37°C overnight. ..

    other:

    Article Title: Nek family members regulate Rad54 during homologous recombination in developing mice
    Article Snippet: H&E images were captured using an Axioimager M1 microscope with ApoTome.2 (Zeiss, Germany) and Metafer4 (MetaSystems) software at 10x and 40x magnification and arranged with Fiji.

    Clone Assay:

    Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation.
    Article Snippet: .. Plasmids were cut with XmaI (New England Biolabs; # R0180S) and PsiI (New England Biolabs; # R0744S), and a geneblock containing the bsd gene was ordered (gb_bsd) and cloned in the cut vector via Gibson assembly in the purified vector for 3 h at 50◦C followed by DH5 α E. coli competent cells (Thermo Fisher Scientific; #18265017). ..



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